|
KMID : 0379119890170010035
|
|
Korean Journal of Mycology
1989 Volume.17 No. 1 p.35 ~ p.38
|
|
|
Cloning and Expression of Leu 2 Gene (β-isopropylmalate dehydrogenase) from the Basidiomycete Flammulina velutipes in E. coli
|
|
박용환/Park, Yong Hwan
유영복/유창현/고승주/변명옥/차동열/Yoo, Young Bok/You, Chang Hyun/Go, Seung Joo/Byun, Myung Ok/Cha, Dong Yul
|
|
|
Abstract
|
|
|
|
Gene libraries of DNA from Flammulina velutipea were constructed using Escherichia coli plasmid pBR 322. Leu 2 gene coding β-isopropylmalate dehydrogenase from F. uelutipes was cloned by complementation of leucine requiring mutant of E. coli. The size of inserted DNA fragment of this clone is about 1 Kbp. The fragment has Bam H1 and Ava I restriction sites.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|